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Yamamoto S, Matsui K, Sasabe M, Ohashi N (2002) Effect of an orally active Na+/H+ exchange inhibitor, SMP-300, on experimental angina and myocardial infarction models in rats

Immunofluorescence staining For most of the immunostaining experiments, cells were fixed with ice-cold methanol for 5 min at 20, washed three times with PBST (0.1% TritonX-100 in PBS) for 5 min and incubated for 30 min with 1% BSA at room temperature

doi: 10.1371/journal.ppat.1007390 209 OnofrioL.CaragliaM.FacchiniG.MargheritaV.De PlacidoS.BuonerbaC

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by the reticulo-endothelial system (RES)