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Eur J Med Res

Each mouse was tested in duplicate through stimulation with RBD protein (10 g/mL), using Concanavalin A (Sigma-Aldrich, No

Excess primary antibody was washed off by three 10 min PBST rinses

Additionally, the long-term effects of DSIP are not fully understood, and it is recommended to consult professionals for safe and ethical research practices

In the context of increased tibial slope, chronic activation of stress-responsive pathways can lead to an imbalance between ECM synthesis and degradation, further compromising the ligaments integrity

The membranes were then incubated in 1% bovine serum albumin (BSA) solution with the following primary antibodies overnight at 4C: anti-AQP4 (Abcam, ab18956), anti-MMP2 (Abcam, ab37150), anti-MMP9 (Abcam, ab38898), anti-TIMP1 (Abcam, ab109125) and anti-beta actin (Abcam, ab8227)