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Cells were exposed to a 1:300 dilution of the primary antibody anti-xCT, in blocking solution overnight at 4 C, followed by the incubation with fluorescein-labeled goat anti-rabbit (1:1000) antibodies for 2 h at room temperature

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Each sample was run with the full MS method for general metabolites and with the parallel reaction monitoring (PRM) method for bromobimane alkylated metabolites

The PD-1blocking antibody is now FDA approved in combination with carboplatin and the chemotherapy paclitaxel followed by Jemperli as a single agent to treat patients with primary advanced or recurrent endometrial cancer

10.1126/scitranslmed.3005123 Sci