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Apple embryonic axes (25 mg) were homogenised in 50 mM K-phosphate buffer (pH 7.5), with 0.1% triton X-100, 5 mM DTT (Sigma, D0632), 5% (w/v) glycerol, 0.1% protease inhibitor cocktail (Sigma, P9599) and 2% (w/v) PVPP on ice

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