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Cell Culture and GSH Preparation K562, HepG2, Hela and L-02 cells were respectively cultured in RPMI 1640 (Hyclone, penicillin 100 U/mL, streptomycin 100 g/mL) adding 10% fetal bovine serum and maintained at 37 C in a humidified atmosphere including 5% CO 2 , in terms of the instructions of the American Type Culture Collection

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The event of interest is considered the GG genotype of GSTP1

IV fluids and nutrients are sourced from FDA-registered pharmacies and prepared in accordance with United States Pharmacopeia (USP) standards, with batch documentation and lot tracking available

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10.1080/15384101.2015.1120930 Cell Cycle 151 PeyronR.Le BarsD.CinottiL.Garcia-LarreaL.GalyG.LandaisP.et al (1994)