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Gibco, Life Technologies), 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin in a humidified incubator at 37 C with 5% CO 2

When choosing a glutathione supplement, look for liposomal formulations, which may enhance absorption and bioavailability

VNS may exert therapeutic effects on various NDDs through the aforementioned mechanisms

Oxidative stress markers The data show that the use of Chlorella , Nannochloropsis, and Arthrospira extracts in concentrations ranging from 0.25% to 1.0% had a significant impact on reducing malondialdhyde (MDA) level, a byproduct of lipid peroxidation caused by free radicals

The Ct method, chosen for its accuracy in quantifying gene expression levels, was employed to analyze gene expression levels, presented as fold-change over untreated controls and normalized to -actin levels

As with all research compounds, investigations should be conducted using proper laboratory practices and established scientific standards