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The insert of pAGB1328 ( hpaA-2xLinker-sfgfp ) was transferred together with the inserts of constructs pAGB249 ( PhrpD ) and pAGB232 ( Xcv term2 ) into the destination vector pICH47742 using Bsa I and ligase and resulting in the level 1 construct pAGB1330 ( PhrpD hpaA-2xLinker-sfgfp )

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In our experimental design, we established a D-galactose-induced aging mouse model and injected the experimental group mice with FOXO4-DRI for 1 month, while the control group was injected with equal amounts of PBS

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