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doi: 10.1016/j.fbio.2024.105274 18

It was shown that BRCA1 occupied the NNMT promoter and regulating its expression ( BRCA1 and CDK12 depletion contribute to mitochondrial respiration defects and decreased ATP levels due to the reduced mitochondrial DNA copy number rather than mitochondrial number, which suggest that they stem from decreased flux through electron transport complexes rather than a decrease in mitochondria (66)

Conditions for each enzymatic reaction are as follows: AmgK: to 100 mM Tris buffer pH 7.9, 2.0 mM of one NAM derivative ( 1 (Sigma-Aldrich) or 2 and 3 ), 4.0 mM ATP and 1.0 mM MgCl 2 was added 1.0 g purified AmgK enzyme per 100 l reaction sample

2021;374:eabh1823