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Cell culture and model establishment Normal human colonic epithelial NCM-460 cells were cultured with RPMI 1640 medium (10% fetal bovine serum) under standard conditions

Involvement of PIAS1 in the sumoylation of tumor suppressor p53

Furthermore, high glucose levels increase the formation of advanced glycation end products (AGEs) through the non-enzymatic reaction between reducing sugars and cellular proteins, lipids, or nucleic acids