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for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein

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In general, minor side effects that may occur with penile injection therapy include: Mild bruising over the site of injection: this can be avoided by pressing over the injection site for 3 minutes (6 minutes if you are using a blood thinner) Penile pain: while uncommon, this occurs more often in men using injections that contain the medicine alprostadil