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doi: 10.1016/S0140-6736(14)60802-3 49 LiY-SLuoWZhuS-ALeiG-H

Lineage-negative cells were enriched using the lineage cell depletion kit (Miltenyi Biotec, 130-110-470), differentiated in the presence of 20 ng ml 1 M-CSF (PeproTech, 315-02) for 3 days, treated with 10 g ml 1 -glucan or PBS for 24 h, cultured in the presence of M-CSF for 3 days, induced with 10 ng ml 1 LPS for 24 h before the medium was collected for quantification of cytokines

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However, for simplicity, compatibility with aqueous solutions, and high yield, acidified nitrite is preferred for biological applications

Chinese hamster V79 cells exposed to MC vapours showed weak positive effect on sister chromatid exchanges [89]