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Approximately 0.51 million colonies were scraped taking care to break colonies apart on the surface of the plates, mixed very thoroughly, aliquoted and stored at 80 C with 20% glycerol, with ~510 l reserved for genomic DNA extraction and reference library preparation

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Then, we used 100 ng of cDNA for the reaction mixture

Human RNA-seq datasets support these observations: large-scale GTEx-based transcriptomic analyses identify the liver as one of the most sexually dimorphic human somatic organs based on metabolic functions, with pronounced sex differences in xenobiotic metabolism, lipid homeostasis, and immune signaling [6, 7]