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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again

BMP7 belongs to a group of adipokines that includes adiponectin, leptin, retinol-binding protein 4 (RBP4), visfatin/nampt/PBEF, bone morphogenetic protein (BMP)-4, fibroblast growth factor 21 (FGF21), cathepsins, nesfatin-1, apelin, vaspin, progranulin, omentin, and lipocalin, among others [258]

TUNEL and immunofluorescence co-staining assay Cell death was detected using the TUNEL FITC Apoptosis Detection Kit (A111-01, Vazyme Biotech) following the manufacturers instructions

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Trata-se na verdade de uma vitamina que pode auxiliar as pessoas a ter a qualidade de vida que apenas um corpo saudvel capaz de proporcionar