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Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

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As a master regulator of immune responses, the nuclear factor-B (NF-B) transcription factor complex governs the expression of numerous inflammation-related genes, including those encoding pro-inflammatory cytokines such as TNF- and interleukin-6 (IL-6), as well as chemokines and adhesion molecules