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Cells or organoids were solubilized in pull-down buffer (50 mM Tris pH 7.3, 150 mM NaCl, 1% Triton X-100, 0.1 mM CaCl 2 plus protease/phosphatase inhibitors as above) and centrifuged at 14,000 rpm for 10 min at 4C

Inhibition of CPS1 or reduction of ammonia levels effectively suppresses collagen production and mitigates fibrosis in experimental models [80]

2023 Feb 3;24(3):2959

Curr Treat Options Oncol (2017) 18(4):23

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Recent advances and upcoming trends in the field are also discussed with a focus on tissue-engineered constructs that could meet metabolic demands to facilitate regeneration