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The cryo-EM samples containing ETAA1 AAD (142 M final concentration) or the ETAA1 AAD mutant (137 M final concentration) were prepared similarly, except the salt composition of the buffer was adjusted to either 100 mM NaCl or a mixture of 50 mM NaCl and 125 mM potassium acetate, which were screened to mitigate protein aggregation on cryo-EM grids

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2 This is a genuinely elegant piece of biochemistry, and it is the reason GHK-Cu is more than a generic peptide: the copper is doing real chemical work

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