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These models exhibit abnormal mitochondrial morphology in neurons, consistent with features of ferroptosis

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Furthermore, there are many negative regulators of ferroptosis in HCC, such as nuclear factor erythroid 2-related factor 2 (NRF2), metallothionein-1G (MT-1G), CDGSH iron-sulfur domain 1 (CISD1) and P53

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The old cell 3-OM did not show an obvious increase of Nrf2 protein upon MB treatment probably due to its extreme senescent cell stage thus limited cells for the analysis