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Thus, while the molecular details of adrenal mitochondrial steroid biosynthesis are well understood, as described below, the evolution of this system remains an important and poorly understood area of research (40)

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Anion exchange elutions were polished by gel filtration using a Superose 6 Hiload 16/600 column (Cytiva) equilibrated in storage buffer (25 mM HEPES, pH 7.5, 500 mM NaCl, 0.5 mM TCEP, 5% glycerol)

doi: 10.3389/fnagi.2020.00150 74 ZhaoN.LiuC

However, our understanding of subcellular NAD(P) redox dynamics is still remarkably incomplete, mainly due to a lack of techniques allowing specific monitoring in defined subcellular compartments in vivo

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