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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol

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Regarding endogenous ACTH and cortisol modelling results, it appeared that 70 kg healthy individuals did not reach maximal cortisol production rate: The quantification of ACTH-dependent cortisol production could help refining and evaluating the results of ACTH stimulation test, a frequently used test to diagnose adrenal insufficiency [30]

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Interplay between superoxide dismutase, glutathione peroxidase, and peroxisome proliferator activated receptor gamma polymorphisms on the risk of end-stage renal disease among Han Chinese patients