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10.3390/antiox14091033 38 GuerraC.MorrisD.SipinA.KungS.FranklinM.GrayD.et al (2011)

In this regard, our study extends beyond the evaluation of inhibiting glutathione synthesis in 3D culture, demonstrating that neutralizing oxidative stress with glutathione supports lymphoid tumor cells proliferation in vivo

NADPH, GSH and GR were mixed in assay buffer before Grx was added and a baseline was recorded for 30 s

Biol Trace Elem Res 82:18

Adding a washing step in cell suspension or 3D cultures also comes with potential drawbacks such as the unavoidable larger quenching time frame ( To extract metabolites, various factors need to be considered, such as the physicochemical properties of metabolites, including their solubility in polar and nonpolar solvents, and their molecular size