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The HPLC conditions employed were a linear 1030% acetonitrile/50 mM TEAA pH 7.0 buffer gradient in 60 min

doi: 10.1093/gerona/glac153 29 Cholewa-WaclawJ.ShahR.WebbS.ChhatbarK.RamsahoyeB.PuschO.et al

After the successful manual optimization of the assays in a plate reader, automated compound library screening was performed against a Spectrum library using a Tecan robotic liquid handling system

an Fc monomer can be a polypeptide constant region comprising a portion of the Ig-Fc hinge region, a CH2 region and a CH3 region, wherein the hinge region is amino terminal to the CH2 domain