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Then cells were diluted with 0.51 ml assay buffer, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)

doi: 10.1016/j.jbc.2024.108047 114 ZhangYMoerkensMRamaiahgariSde BontHPriceLMeermanJet al

BPC-157 showed no adverse effects even under prolonged exposure

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Phage display library screening Immunoplate strips (SPL Life Science) were coated with 1 g of the target protein and blocked with 3% bovine serum albumin (BSA) in Tris-buffered saline (TBS)

Inhibition of SOD1 by the potent inhibitor ATN-224 (bis (choline)tetrathiomolybdate) increases intracellular O 2 levels, which unexpectedly results in the suppression of glutathione peroxidase (GPx) activity, leading to a significantly increased concentration of intracellular H 2 O 2