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Then, differential expression analysis was performed using DEseq2 (v.1.4.5) and gene expression differences between groups highlighted in volcano plots

The beads were washed twice in 500 l citrate-phosphate buffer, and once in immunoprecipitation (IP) lysis buffer (0.3% (v/v) NP-40, 200 mM NaCl, 50 mM Tris, pH 7.4, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1 mM EGTA, with freshly added 1 mM sodium vanadate, 50 mM sodium fluoride and a protease-inhibitor cocktail)

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Nuclear lamina erosion-induced resurrection of endogenous retroviruses underlies neuronal aging

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