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Human-derived cells were cultured in DMEM medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS

For comparison, Kunming mice died within 1 h after intravenous administration of Lipo 2000 and GO-CQDs at doses of 12 and 16 mg kg 1 , respectively

For instance, roGFP2-Orp1, the fusion of roGFP2 to the thiol peroxidase enzyme Orp1 is usually employed as a HO-sensitive probe and can be used to determine how chemicals induce or drugs decrease production of ROS in cells (AN252: Real-time monitoring of genetically encoded redox probes in mammalian cell monolayers)

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Oxidative stress occurs when there is too many free radicals or too few antioxidants

Acetylcysteine (acetylcysteine) Indication: Paracetamol poisoning