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Under these chromatographic conditions, 20 L of each sample, composed of ACN/H 2 O (65/35) in the mobile phase, was injected into the isocratic module at a flow of 0.7 mL.min -1

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To incorporate such in silico predictions in a comprehensive carrier design framework, one needs: Direct engagement of target validation (competitive binding assays, cellular thermal shift assays, or kinase activity) for confirmation that naringenin effectively engages these targets at therapeutically achievable concentrations in vivo

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The disruption of protein synthesis results in the production of non-functional or toxic peptides, leading to bacterial cell death ( Amikacin exerts bactericidal effects against both Gram-positive and Gram-negative bacteria, including strains resistant to other aminoglycosides like gentamicin and tobramycin