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As a result of this damage, chromatin remodelling is impaired, and incomplete protamination of sperm DNA increases susceptibility to oxidative damage, making DNA more prone to fragmentation [148, 154]

You can resume normal activities immediately

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Additionally, heavy alcohol use can strain the liver and other organs, complicating overall treatment

Buffer conditions were as follows: Binding buffer: 50 mM NaH 2 PO 4 , 150 mM NaCl and 10 mM Imidazole, pH 7.4 Elution buffer: 50 mM NaH 2 PO 4 , 150 mM NaCl and 100 mM imidazole, pH 7.4 On purification, the protein co-elutes with chaperones and non-specific bands

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