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doi:10.3390/ijms21218289 392. Dentillo DB, Meola J, Rosa e Silva JC, Giuliatti S, Silva Junior WA Jr., Ferriani RA, Martelli L

TaqMan Gene Expression Assay (Applied Biosystems, Termo Fisher Scientific, Waltham, MA, USA) hydrolysis probes were used for gene expression analysis of selected genes ( Table 1 ) with TaqMan Gene Expression Master Mix (Applied Biosystems, Termo Fisher Scientific, Waltham, MA, USA)

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have demonstrated equivalent activity after lyophilization and reconstitution of O 2 -tolerant [NiFe]-hydrogenases to freshly isolated enzyme, indicating that metal cofactors and amino acid side-chains responsible for proton/electron transfer were not altered by lyophilization 36

In such cases, the remaining amount of nitric oxide after its reaction with the test sample is quantified as nitrite

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