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Description

The forward and reverse oligonucleotides of this fragment were, respectively, 5- GATCT ATGCTGTTTAATCTGAGGATCCTGTTAAACAATGCAGCTTTTAGAAATGGTCACA ACTTCATGGTTCGAAATTTTCGGTGTGGACAACCACTACAAG-3 and 5- AATTC TTGTAGTGGTTGTCCACACCGAAAATTTCGAACCATGAAGTTGTGACCATTTCTA AAAGCTGCATTGTTTAACAGGATCCTCAGATTAAACAGCATA-3 ( Bgl II and Eco RI sites underlined

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Koh, K.K., Park, S.M

Vet Res 47(1):112

For deeper nutrient repletion, especially for chronic conditions, the effects may be more gradual, with positive changes noticed over the next few hours or days