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Besides, to further confirm peroxynitrite-mediated activation of the NF-B pathway, we also measured the mRNA expression levels of known NF-B p65 target genes Bax , P53 , Bcl2 , and Bcl-xL

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In stark contrast, the massive and uncontrolled oxidative burst during reperfusion overwhelms these delicate regulatory systems, causing widespread, indiscriminate damage to lipids, proteins, and nucleic acids, which directly drives myocardial injury (Fedoreyeva, 2024)