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Li D, Wang WJ, Wang YZ, Wang YB, Li YL

An EM GP cryopreparation system (Leica Microsystems, Buffalo Grove, IL) was used for plunge-freezing with the following parameters: 5 s pre-blot time, 2.05.0 s blotting time, followed by 0.5 s post-blot time

In intestinal tissues, IR decreases GSH levels and inhibits GPX4 expression, thereby promoting ferroptosis [39]

Antioxidant mechanisms are regulatory systems that inhibit ferroptosis System Xc /GSH/GPX4 axis: The system Xc is an input cystine/output glutamate reverse transport system, which includes two subunits, SLC3A2 and SLC7A11 (Koppula et al., 2021b)

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This susceptibility poses a serious threat to human health, contributing to approximately 2 million deaths annually, accounting for 4% of global mortality (1)