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In vitro binding assay 3 pmol factor-free 80S with 24 pmol GST-NMT1 and 24 pmol NAC-wt, -mutants or individual components were incubated in Binding Buffer (20 mM HEPES/KOH pH 7.5, 100 mM KOAc, 5 mM MgCl 2 , 2 mM DTT) for 1 h on ice

The amount of ATP is directly proportional to the number of cells present

doi: 10.1079/WPS20030006 39 DodsonM

Antihistamine side effects arise from their anticholinergic action and include redness of the skin, tachycardia, hyperthermia, blurred vision, drowsiness, delirium, hallucinations, seizures, and death

Mol Pharmacol 78(4):723733