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When the column temperature is reduced to 25C, the retention time becomes 33.9 minutes, indicating that the compound of lower temperatures eluted at a slower rate than at the other temperature

The decision to submit the manuscript for publication was made by the authors

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Wherein freeze-dry process is: Shelf temperature is established below-40 and is carried out pre-freeze, and products temperature kept 180 minutes at-40 , and case vacuum value reached below the 15pa before evacuation made

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