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Antitumoral activities of curcumin and recent advances to improve its oral bioavailability

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Right panel, quantification of p21 Cip1 IHC staining in PC3 tumours

Adult mouse astrocyte isolation Brain tissues were aseptically removed, minced and enzymatically digested in HBSS (Gibco, 14175103) containing 50 mg ml 1 collagenase D (Sigma-Aldrich, 11088858001), 100 g ml 1 TLCK trypsin inhibitor (Sigma-Aldrich, T9378), 100 U l 1 DNase I (Sigma-Aldrich, D5025), and HEPES pH 7.2 (Gibco, 15630080) for 1 h at 23 C with shaking

Over a period of 50 days, 11 postmenopausal women received six different treatments: soy cotyledon (the first leaves to appear on the plant), soy germ, kudzu (a plant used in traditional and folk medicine), red clover, risedronate (a drug used to treat osteoporosis), and estrogen combined with progesterone