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Lee) polysaccharides promote the longevity of Caenorhabditis elegans through autophagy-dependent insulin and mitochondrial pathways

This transformation was evidenced by the upregulation of the browning genes Ucp1 and Prdm16 , activation of HSL (p-HSL 660 ), and increased glycerol and free fatty acid release

This occurs via the lipid metabolism hub gene Phospholipase A2 Group IIA in homoeostatic chondrocytes, a Phospholipase A2 enzyme that promotes OA pathology by increasing arachidonic acid release and activating downstream pro-inflammatory pathways (Wang Y

First the vector was digested in a reaction with Fast Digest Buffer (B64, Thermo Fisher Scientific), BsmBI restriction enzyme (FD0454, Thermo Fisher Scientific), DTT (10 mM) with program 45 min at 37 C, heat inactivate 10 min at 65 C

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