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Protein concentration was determined by a BCA assay according to the manufacturers protocol

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Finally, HLPC was used to measure GSH levels in the aqueous humour and lenses every 4 hours over a 24-hour period

Interestingly, P62 did not decrease in the H/R group, which accumulated due to the impaired fusion of autophagosomeslysosomes, demonstrating H/R-impaired autophagy flux