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But, the thermostability of lactuca sativa GST was investigated by incubation the enzyme at various temperature for 10 minutes, the midpoint of the temperature stability curve was approximately 53C for the enzyme

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And (3) dripping a cyclization reagent until the color of the cyclization solution is not faded after the solution is stirred for about 5 minutes, and dripping a proper amount of Vc reagent until the cyclization solution is clear and colorless

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