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The samples were subjected to standard SDS-PAGE on 12% polyacrylamide gels with a 5% stacking gel 27

[5] previously evaluated the separate roles of insulin and IGF-1 in directly regulating GH secretion, using the Cre/loxP system to knock down the insulin receptor (INSR) and IGF-1 receptor (IGFIR) in primary mouse pituitary cell cultures

Glutathione supplements cannot enter cells as there is an insurmountable concentration gradient across the cell membrane and, although there are well-understood membrane transporters for transporting glutathione from the inside to the outside of the cell, therefore most cell types, there are no transporters for transporting extracellular glutathione in the reverse direction i.e

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doi: 10.1371/journal.pone.0111898 141 IzzoN