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Specifically, a variety of primary amine substrates ( 2ag ) were coupled with intermediate 1 in neutral aqueous solution to furnish the corresponding 3Br-5MPs ( 3ag ) in modest yields (Method 1 in Table 1)

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After incubation at 37 C for 24 h, the cells on the surface of the membrane were washed with PBS, fixed with 4% paraformaldehyde for 15 min and stained with 0.1% crystal violet for 30 min at room temperature

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