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Chemicals and materials A range of essential laboratory reagents such as distilled water, EDTA-coated collection tubes, cold saline, 4% neutral buffered formalin, ice-cold PBS (pH 7.4), ethanol, xylene, paraffin wax, hematoxylin and eosin (H&E) stains, carboxymethylcellulose (CMC), and olive oil were either prepared on-site or obtained from general laboratory suppliers, with some sourced from commercial vendors within Egypt

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