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Next, cDNA was synthesized by reverse transcription using the QuantiTect Reverse Transcription Kit from Qiagen according to the manufacturers instructions, and qRT-PCR was finally performed using a QuantiTect SYBR Green PCR Kit and QuantiTect Primer Assays (Qiagen) on a LightCycler 480 instrument (Roche)

Advantages of 2-O-methyl oligoribonucleotide probes for detecting RNA targets

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While there is limited information on how other NF-B signaling intermediates affect T cell metabolism, some studies suggest a link between NF-B signaling and mTOR in regulating metabolic changes critical for T cell differentiation (197, 198)

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