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Description

Since the refolding procedure that is required to obtain disulfide containing proteins in their native state can lead to loss of the thioester functionality, NCL is typically performed directly on the immobilized fusion protein to yield a stable amide bond, followed by refolding in redox buffer systems [18, 19]

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Zhao, L., Mechanism of Natural Drugs on Nonalcoholic Fatty Liver Disease

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Aptamers, antibody scFv, and antibody fab fragments: an overview and comparison of three of the most versatile biosensor biorecognition elements

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