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C.LanchoO.DuckerG

A tripartite (GSTP1-G6PD-SRC) complex is modulated by lactic acid We ectopically expressed Flag-tagged GSTP1 in three cell lines (MCF-7, Bcap37, and 293 T) and used cell lysates mixed with M2 beads to immuno-precipitate (IP) tagged GSTP1 with or without lactic acidosis

Helium was used as a carrier gas at a flow rate of 1.1 ml min 1

Transl Cancer Res 8(4):10651073

Pyruvate, on the other hand, is an end product of glycolysis that becomes oxidized in the tricarboxylic acid (TCA) cycle within mitochondria, ultimately feeding into oxidative phosphorylation