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4.4.1 Glutamine Glutamine metabolism not only promotes the synthesis of succinic acid by fueling the TCA cycle in M1-type macrophages, but also drives M2 polarization via the conversion to glutamate and then -KG, with high -KG levels and a high -KG/succinate ratio facilitating macrophage reprogramming towards the M2 phenotype (Liu et al., 2017)

Using an established assay for CRISPR-based knock-in of sequences at the CLTA N-terminus (a highly expressed gene in HEK293T cells [Leonetti 2016] , neither our original split-wrmScarlet 11 sequence nor its mutant library enabled detectable complementation (Figure S10B, left panels)

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(2/)ROS-targeting therapies were tested