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Baskin L, Sinclair A, Derpinghaus A, Cao M, Li Y, Overland M, Aksel S, Cunha GR (2021) Estrogens and development of the mouse and human external genitalia

Briefly, to assess GSH in the cells, a non-fluorescent substrate, monochlorobimane (mBCl, Thermo Fisher Scientific Inc., Cat No

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Bound mt-hNNMT was eluted from the column with lysis buffer and 150 mM imidazole, 200 mM salt, and 5% glycerol in 1 ml aliquots