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Aminoglycosides, tetracyclines, and certain other antibiotics may alter gut microbiota composition, potentially affecting the small amount of vitamin B12 that is produced by colonic bacteria, though this is generally not clinically significant for patients receiving injectable B12 supplementation

Critics, including the FDA's own scientific staff, argue that insufficient evidence exists to ensure these compounds are safe for human use

It was shown that BRCA1 occupied the NNMT promoter and regulating its expression ( BRCA1 and CDK12 depletion contribute to mitochondrial respiration defects and decreased ATP levels due to the reduced mitochondrial DNA copy number rather than mitochondrial number, which suggest that they stem from decreased flux through electron transport complexes rather than a decrease in mitochondria (66)

During the low-dose titration stages, you will use far less than 5mg in 30 days

You will have to figure out what your body does best on, but in general, I recommend using 5,000mcg per day in sublingual form as long as you are not extremely sensitive to supplements and/or medications