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SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism

coli Rosetta2DE3pLysS cells transformed with recombinant plasmids were grown to an A 600 of 1.0 at 30 C and the protein induction was carried out at 18 C for 12 h using 1 mM isopropyl--D-thiogalactoside (IPTG) (MP Biomedicals, 11IPTG0001)

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