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sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml

Vitamin C (L-Ascorbic Acid and derivatives): A potent antioxidant, Vitamin C is celebrated for its ability to brighten the complexion, reduce the appearance of dark spots and hyperpigmentation, and protect the skin from environmental damage caused by free radicals

Compared to placebo, both oils significantly improved all skin properties measured

Schulze RJ, Sathyanarayan A, Mashek DG

10.1111/exd.13548 5 Carvajal-YepesM.HimmelsbachK.SchaedlerS.PloenD.KrauseJ.LudwigL.et al (2011)