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FFWO assay Vero cells were seeded in 24-well plates, washed once with RPMI 1640 supplemented with 0.2% BSA, 10 mM HEPES pH 7.4, and 20 mM NH 4 Cl, and incubated for 15 min at 4 C

We hypothesised that linker CCA could selectively conjugate to the peptide cysteine residues whilst sparing those found within pIII, because of the selectivity and fast kinetics of the reaction of CPO with the N-terminal cysteine

Furthermore, the concentration of amino acids in your blood is a primary trigger for protein synthesis

To view a copy of this licence, visit About this article Cite this article Fang, X., Zhao, H., Ke, W

Arch Virol 154:775781