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The 100 L serum of every sample was positioned in a 96-well plate and mixed by 300 L of precooled extract solution (methanol: acetonitrile, 2:1, v/v)

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Published in-vitro work commonly uses GHK-Cu in the range of 10 nM to 10 M, with 1 M as a frequently reported working concentration

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2.13 Liver and kidney function tests Serum alanine aminotransferase enzyme (ALT), aspartate aminotransferase enzyme (AST), creatinine, urea, Na, and K were measured using the colorimetric method with commercial kits specifically designed for laboratory use from Biodiagnostic, Diagnostic, and Research Reagents, Cairo, Egypt