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The herbs were dried at room temperature and stored at 4 C until use

however, GSH levels are also influenced by agents or conditions that alter the thiol redox state, that lead to the formation of glutathione S -conjugates or complexes, and/or that disrupt the distribution of GSH among various intracellular organelles

These data emphasize the controversial effects of ROS and careful considerations in Nox inhibitor development aiming to reduce ROS levels

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Tumor cells have increased iron ion content compared to normal cells, and activation of Nrf2 can HO-1 activation, while excessive activation of HO-1 can cause cellular iron overload, induce cellular ferroptosis and exert cytotoxicity